Comprehensive characterization of the antibody responses to SARS-CoV-2 Spike protein after infection and/or vaccination.

ABSTRACT:

Background

Control of the COVID-19 pandemic will rely on SARS-CoV-2 vaccine-elicited antibodies to protect against emerging and future variants; an understanding of the unique features of the humoral responses to infection and vaccination, including different vaccine platforms, is needed to achieve this goal.

Methods

The epitopes and pathways of escape for Spike-specific antibodies in individuals with diverse infection and vaccination history were profiled using Phage-DMS. Principal component analysis was performed to identify regions of antibody binding along the Spike protein that differentiate the samples from one another. Within these epitope regions we determined potential escape mutations by comparing antibody binding of peptides containing wildtype residues versus pep

SUBMITTER: Garrett ME 

PROVIDER: S-EPMC8509098 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature