Understanding the Adsorption of Peptides and Proteins onto PEGylated Gold Nanoparticles.

ABSTRACT: Polyethylene glycol (PEG) surface conjugations are widely employed to render passivating properties to nanoparticles in biological applications. The benefits of surface passivation by PEG are reduced protein adsorption, diminished non-specific interactions, and improvement in pharmacokinetics. However, the limitations of PEG passivation remain an active area of research, and recent examples from the literature demonstrate how PEG passivation can fail. Here, we study the adsorption amount of biomolecules to PEGylated gold nanoparticles (AuNPs), focusing on how different protein properties influence binding. The AuNPs are PEGylated with three different sizes of conjugated PEG chains, and we examine interactions with proteins of different sizes, charges, and surface cysteine content. The expe

SUBMITTER: Perera YR 

PROVIDER: S-EPMC8510204 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature