Genome-Wide Identification of Ralstonia solanacearum Genes Required for Survival in Tomato Plants.

ABSTRACT: Ralstonia solanacearum is an extremely destructive phytopathogenic bacterium for which there is no effective control method. Though many pathogenic factors have been identified, the survival strategies of R. solanacearum in host plants remain unclear. Transposon insertion sequencing (Tn-seq) is a high-throughput genetic screening technology. This study conducted a Tn-seq analysis using the in planta environment as selective pressure to identify R. solanacearum genes required for survival in tomato plants. One hundred thirty genes were identified as putative genes required for survival in tomato plants. Sixty-three of these genes were classified into four Clusters of Orthologous Groups categories. The absence of genes that encode the outer membrane lipoprotein LolB (RS_RS01965) or th

SUBMITTER: Su Y 

PROVIDER: S-EPMC8510521 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature