Probing interactions of therapeutic antibodies with serum via second virial coefficient measurements.

ABSTRACT: Antibody-based therapeutics are the fastest-growing drug class on the market, used to treat aggressive forms of cancer, chronic autoimmune conditions, and numerous other disease states. Although the specificity, affinity, and versatility of therapeutic antibodies can provide an advantage over traditional small-molecule drugs, their development and optimization can be much more challenging and time-consuming. This is, in part, because the ideal formulation buffer systems used for in vitro characterization inadequately reflect the crowded biological environments (serum, endosomal lumen, etc.) that these drugs experience once administered to a patient. Such environments can perturb the binding of antibodies to their antigens and receptors, as well as homo- and hetero-aggregation, thereby alte

SUBMITTER: Larsen HA 

PROVIDER: S-EPMC8510860 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature