Efficient marmoset genome engineering by autologous embryo transfer and CRISPR/Cas9 technology.

ABSTRACT: Genetic engineering of non-human primates, which are most closely related to humans, has been expected to generate ideal animal models for human genetic diseases. The common marmoset (Callithrix jacchus) is a non-human primate species adequate for the production of genetically modified animals because of their small body size and high reproductive capacity. Autologous embryo transfer (AET) is routinely utilized in assisted reproductive technologies for humans but not for experimental animals. This study has developed a novel method for efficiently producing mutant marmosets using AET and CRISPR/Cas9 systems. The embryos were recovered from oviducts of naturally mated females, injected with Cas9/guide RNA, and transferred into the oviducts of the donors. This AET method can reduce the time

SUBMITTER: Abe Y 

PROVIDER: S-EPMC8511084 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature