Restriction of RecG translocation by DNA mispairing.

ABSTRACT:

Background

The RecG DNA helicase plays a crucial role in stalled replication fork rescue. We have recently discovered that interaction of RecG with single-strand DNA binding protein (SSB) remodels RecG, allowing it to spontaneously translocate upstream of the fork. Based on these findings, we hypothesized that mispairing of DNA could limit such translocation of RecG.

Methods

Here, we used atomic force microscopy (AFM) to directly test this hypothesis and investigate how sensitive RecG translocation is to different types of mispairing.

Results

We found that a CC mispairing, at a distance of 30 bp from the fork position, prevents translocation of RecG over this mispairing. A G-bulge, placed at the same distance, also has a similar blocking efficiency. However, a CC misp

SUBMITTER: Sun Z 

PROVIDER: S-EPMC8511092 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature