The N-terminus of MIF regulates the dynamic profile of residues involved in CD74 activation.

ABSTRACT: Macrophage migration inhibitory factor (MIF) is an immunomodulatory protein with a pathogenic activity in various inflammatory disorders, autoimmune diseases, and cancer. The majority of MIF-triggered pathological conditions are associated with activation of the cell surface receptor CD74. In the absence of small molecule antagonists that directly target CD74, MIF variants and MIF-ligand complexes have served as modulators of CD74 activity. These molecules have been reported to have either antagonistic or agonistic effects against the receptor, although the mechanistic parameters that distinguish the two groups are largely unknown. Through molecular dynamics simulations and NMR experiments, we explored the relationship between MIF's catalytically active N-terminus and the surface residues

SUBMITTER: Parkins A 

PROVIDER: S-EPMC8511117 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature