Monitoring the effects of chemical stimuli on live cells with metasurface-enhanced infrared reflection spectroscopy.

ABSTRACT: Infrared spectroscopy has found wide applications in the analysis of biological materials. A more recent development is the use of engineered nanostructures - plasmonic metasurfaces - as substrates for metasurface-enhanced infrared reflection spectroscopy (MEIRS). Here, we demonstrate that strong field enhancement from plasmonic metasurfaces enables the use of MEIRS as a highly informative analytic technique for real-time monitoring of cells. By exposing live cells cultured on a plasmonic metasurface to chemical compounds, we show that MEIRS can be used as a label-free phenotypic assay for detecting multiple cellular responses to external stimuli: changes in cell morphology, adhesion, and lipid composition of the cellular membrane, as well as intracellular signaling. Using a focal plane ar

SUBMITTER: Huang SH 

PROVIDER: S-EPMC8511245 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature