Long-read sequencing reveals the structural complexity of genomic integration of HBV DNA in hepatocellular carcinoma.

ABSTRACT: The integration of HBV DNA into the human genome can disrupt its structure in hepatocellular carcinoma (HCC), but the complexity of HBV genomic integration remains elusive. Here we applied long-read sequencing to precisely elucidate the HBV integration pattern in the human hepatocellular genome. The DNA library was sequenced using the long-read sequencing on GridION and PacBio Sequel II, respectively. The DNA and mRNA were sequenced using next-generation sequencing on Illumina NextSeq. BLAST (Basic Local Alignment Search Tool) and local scripts were used to analyze HBV integration patterns. We established an analytical strategy based on the long-read sequences, and analyzed the complexity of HBV DNA integration into the hepatocellular genome. A total of 88 integrated breakpoints were ident

SUBMITTER: Zhuo Z 

PROVIDER: S-EPMC8511263 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature