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A Galactosidase-Activatable Fluorescent Probe for Detection of Bacteria Based on BODIPY.

ABSTRACT: Pathogenic E. coli infection is one of the most widespread foodborne diseases, so the development of sensitive, reliable and easy operating detection tests is a key issue for food safety. Identifying bacteria with a fluorescent medium is more sensitive and faster than using chromogenic media. This study designed and synthesized a β-galactosidase-activatable fluorescent probe BOD-Gal for the sensitive detection of E. coli. It employed a biocompatible and photostable 4,4-difluoro-3a,4a-diaza-s-indancene (BODIPY) as the fluorophore to form a β-O-glycosidic bond with galactose, allowing the BOD-Gal to show significant on-off fluorescent signals for in vitro and in vivo bacterial detection. This work shows the potential for the use of a BODIPY based enzyme substrate for pathogen detection.

SUBMITTER: Chen X

PROVIDER: S-EPMC8512000 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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A Galactosidase-Activatable Fluorescent Probe for Detection of Bacteria Based on BODIPY.

Chen Xi X Liu Yu-Cong YC Cui Jing-Jing JJ Wu Fang-Ying FY Xiao Qiang Q

Molecules (Basel, Switzerland) 20211008 19

Pathogenic E. coli infection is one of the most widespread foodborne diseases, so the development of sensitive, reliable and easy operating detection tests is a key issue for food safety. Identifying bacteria with a fluorescent medium is more sensitive and faster than using chromogenic media. This study designed and synthesized a β-galactosidase-activatable fluorescent probe BOD-Gal for the sensitive detection of E. coli. It employed a biocompatible and photostable 4,4-diflu ...[more]

PMID: 34641615

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