Immunomodulatory Activities of Ammodytes personatus Egg Lipid in RAW264.7 Cells.

ABSTRACT: Ammodytes personatus, known as the Pacific sand lance, thrives in cold areas of the North Pacific. In this study, the total lipid was extracted from A. personatus eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID). The results showed that the extracted lipid contained high content of polyunsaturated fatty acids (PUFAs). The immunomodulatory activities of the A. personatus lipid were investigated using rodent macrophages. First, immune enhancement was analyzed, and the A. personatus lipid significantly and dose-dependently increased the NO production in RAW264.7 cells, and this lipid also regulated the transcription of immune-associated genes in RAW264.7 cells by activating the NF-κB and MAPK pathways. Additionally, flow cytomet

SUBMITTER: Lim JH 

PROVIDER: S-EPMC8512018 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature