Study on the CID Fragmentation Pathways of Deprotonated 4'-Monophosphoryl Lipid A.

ABSTRACT: Lipid A, the membrane-bound phosphoglycolipid component of bacteria, is held responsible for the clinical syndrome of gram-negative sepsis. In this study, the fragmentation behavior of a set of synthetic lipid A derivatives was studied by electrospray ionization multistage mass spectrometry (ESI-MSⁿ), in conjunction with tandem mass spectrometry (MS/MS), using low-energy collision-induced dissociation (CID). Genealogical insight about the fragmentation pathways of the deprotonated 4'-monophosphoryl lipid A structural analogs led to proposals of a number of alternative dissociation routes that have not been reported previously. Each of the fragment ions was interpreted using various possible mechanisms, consistent with the principles of reactions described in organic chemistry. S

SUBMITTER: Aissa I 

PROVIDER: S-EPMC8512036 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature