Ontology highlight
ABSTRACT:
SUBMITTER: Gilmutdinov R
PROVIDER: S-EPMC8513165 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Gilmutdinov Rudolf R Kozlov Eugene N EN Yakovlev Konstantin V KV Olenina Ludmila V LV Kotov Alexei A AA Barr Justinn J Zhukova Mariya M Schedl Paul P Shidlovskii Yulii V YV
Development (Cambridge, England) 20210909 17
CPEB proteins are conserved translation regulators involved in multiple biological processes. One of these proteins in Drosophila, Orb2, is a principal player in spermatogenesis. It is required for meiosis and spermatid differentiation. During the later process, orb2 mRNA and protein are localized within the developing spermatid. To evaluate the role of the orb2 mRNA 3'UTR in spermatogenesis, we used the CRISPR/Cas9 system to generate a deletion of the orb2 3'UTR, orb2R. This deletion disrupts t ...[more]