EF24 exerts cytotoxicity against NSCLC via inducing ROS accumulation.

ABSTRACT:

Background

The role of Diphenyldifluoroketone (EF24), a synthetic analogue of curcumin with noteworthy antitumor potential, remains unclear in non-small cell lung cancer (NSCLC). Herein, the inhibitory effect of EF24 on NSCLC and its mechanism were studied.

Methods

Cytotoxicity was measured by MTT assay, colony formation assay and xenograft model. Cell apoptosis and reactive oxygen species (ROS) level were quantified by flow cytometer. Protein level was detected by western blot assay. Mitochondria and autophagosomes were observed using transmission electron microscope and confocal microscopy.

Results

In-vitro, EF24 significantly induced proliferation inhibition, apoptosis, mitochondrial fission and autophagy of NSCLC cell lines. These cytotoxic effects were significan

SUBMITTER: Chang M 

PROVIDER: S-EPMC8513219 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature