An SETD1A/Wnt/β-catenin feedback loop promotes NSCLC development.

ABSTRACT:

Background

SETD1A, a member of SET1/MLL family H3K4 methyltransferases, is involved in the tumorigenesis of numerous cancers. However, the biological role and mechanism of SETD1A in non-small cell lung cancer (NSCLC) remain to be elucidated.

Methods

The expression of SETD1A, NEAT1, EZH2, and β-catenin in NSCLC tissues and cell lines was detected by qRT-PCR, immunohistochemistry and western blotting. The regulatory mechanisms were validated by chromatin immunoprecipitation, co-immunoprepitation and luciferase reporter assay. The self-renewal, cisplatin sensitivity and tumorigenesis of NSCLC cells were analyzed using sphere formation, CCK-8, colony formation assays and xenograft tumor models.

Results

SETD1A expression was significantly increased in NSCLC and its overexp

SUBMITTER: Wang R 

PROVIDER: S-EPMC8513302 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature