A novel gene expression signature-based on B-cell proportion to predict prognosis of patients with lung adenocarcinoma.

ABSTRACT:

Background

This study aimed to develop a reliable immune signature based on B-cell proportion to predict the prognosis and benefit of immunotherapy in LUAD.

Methods

The proportion of immune cells in the TCGA-LUAD dataset was estimated using MCP-counter. The Least Absolute Shrinkage and Selector Operation was used to identify a prognostic signature and validated in an independent cohort. We used quantitative reverse transcription-polymerase chain reaction (qRT-PCR) data and formalin-fixed paraffin-embedded (FFPE) specimens immunohistochemistry to illustrate the correlation between prognostic signature and leukocyte migration.

Results

We found that the relative abundance of B lineage positively correlated with overall survival. Then, we identified a 13-gene risk-score p

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8513350 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature