Development of a physiological model of human middle ear epithelium.

ABSTRACT:

Introduction

Otitis media is an umbrella term for middle ear inflammation; ranging from acute infection to chronic mucosal disease. It is a leading cause of antimicrobial therapy prescriptions and surgery in children. Despite this, treatments have changed little in over 50 years. Research has been limited by the lack of physiological models of middle ear epithelium.

Methods

We develop a novel human middle ear epithelial culture using an air-liquid interface (ALI) system; akin to the healthy ventilated middle ear in vivo. We validate this using immunohistochemistry, immunofluorescence, scanning and transmission electron microscopy, and membrane conductance studies. We also utilize this model to perform a pilot challenge of middle ear epithelial cells with SARS-CoV-2.

Resul

SUBMITTER: Mather MW 

PROVIDER: S-EPMC8513425 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature