Biochemical and Mechanical Gradients Synergize To Enhance Cartilage Zonal Organization in 3D.

ABSTRACT: Articular cartilage is characterized by zonal organizations containing dual gradients of biochemical cues and mechanical cues. However, how biochemical gradient interacts with the mechanical gradient to drive the cartilage zonal development remains largely unknown. Here, we report the development of a dual-gradient hydrogel platform as a 3D niche to elucidate the relative contributions of biochemical and mechanical niche gradients in modulating zonal-specific chondrocyte responses and cartilage zonal organization. Chondroitin sulfate (CS), a major constituent of cartilage extracellular matrix, was chosen as the biochemical cue. Poly(ethylene glycol), a bioinert polymer, was used to create the stiffness gradient. Dual-gradient hydrogels upregulated cartilage marker expressions and increased

SUBMITTER: Zhu D 

PROVIDER: S-EPMC8513795 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature