Leveraging an enzyme/artificial substrate system to enhance cellular persulfides and mitigate neuroinflammation.

ABSTRACT: Persulfides and polysulfides, collectively known as the sulfane sulfur pool along with hydrogen sulfide (H₂S), play a central role in cellular physiology and disease. Exogenously enhancing these species in cells is an emerging therapeutic paradigm for mitigating oxidative stress and inflammation that are associated with several diseases. In this study, we present a unique approach of using the cell's own enzyme machinery coupled with an array of artificial substrates to enhance the cellular sulfane sulfur pool. We report the synthesis and validation of artificial/unnatural substrates specific for 3-mercaptopyruvate sulfurtransferase (3-MST), an important enzyme that contributes to sulfur trafficking in cells. We demonstrate that these artificial substrates generate persulfides <

SUBMITTER: Bora P 

PROVIDER: S-EPMC8513928 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature