The transcription factor Xrp1 is required for PERK-mediated antioxidant gene induction in Drosophila.

ABSTRACT: PERK is an endoplasmic reticulum (ER) transmembrane sensor that phosphorylates eIF2α to initiate the Unfolded Protein Response (UPR). eIF2α phosphorylation promotes stress-responsive gene expression most notably through the transcription factor ATF4 that contains a regulatory 5' leader. Possible PERK effectors other than ATF4 remain poorly understood. Here, we report that the bZIP transcription factor Xrp1 is required for ATF4-independent PERK signaling. Cell-type-specific gene expression profiling in Drosophila indicated that delta-family glutathione-S-transferases (gstD) are prominently induced by the UPR-activating transgene Rh1^G69D. Perk was necessary and sufficient for such gstD induction, but ATF4 was not required. Instead, Perk an

SUBMITTER: Brown B 

PROVIDER: S-EPMC8514241 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature