TP53 signature diagnostic system using multiplex reverse transcription-polymerase chain reaction system enables prediction of prognosis of breast cancer patients.

ABSTRACT:

Background

TP53 status based on TP53 signature, a gene expression profile to determine the presence or absence of TP53 mutation, is an independent prognostic factor of breast cancer. The purpose of this study was to develop a simple diagnostic system for TP53 signature status.

Methods

We developed a multiplex reverse transcription-polymerase chain reaction system to determine TP53 status. Based on this system, prospectively collected 189 patients with stage I and II breast cancer were determined to have TP53 mutant signature or TP53 wild-type signature. The prognostic significance of the TP53 signature by the diagnostic system was analyzed.

Results

The diagnostic accuracy of TP53 status and reproducibility of this diagnosis system was confirmed. Using the diagnostic s

SUBMITTER: Takahashi S 

PROVIDER: S-EPMC8514380 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature