Tetraspanin CD82 regulates S1PR₁-mediated hematopoietic stem and progenitor cell mobilization.

ABSTRACT: Hematopoietic stem and progenitor cell (HSPC) mobilization into the blood occurs under normal physiological conditions and is stimulated in the clinic to enable the isolation of HSPCs for transplantation therapies. In the present study, we identify the tetraspanin CD82 as a novel regulator of HSPC mobilization. Using a global CD82 knockout (CD82KO) mouse, we measure enhanced HSPC mobilization after granulocyte-colony stimulating factor (G-CSF) or AMD3100 treatment, which we find is promoted by increased surface expression of the sphingosine 1-phosphate receptor 1 (S1PR₁) on CD82KO HSPCs. Additionally, we identify a disruption in S1PR₁ internalization in CD82-deficient HSPCs, suggesting that CD82 plays a critical role in S1PR₁ surface regulation. Finally, co

SUBMITTER: Saito-Reis CA 

PROVIDER: S-EPMC8514849 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature