Conjoint application of ultrasonication and redox pair mediated free radical method enhances the functional and bioactive properties of camel whey-quercetin conjugates.

ABSTRACT: Ultrasonication, redox-pair generated free radical method and their combination (Ultrasonication/redox-pair method) was used for production of camel whey-quercetin conjugates. FTIR and SDS-PAGE confirmed successful production of whey-quercetin conjugates using ultrasonication and ultrasonication/redox-pair method. FTIR suggested existence of covalent (appearance of new peak at 3399 cm^-1) and non-covalent linkages (shifting of peak at 3271 cm^-1, 1655 cm^-1 (amide I), 1534 cm^-1 and 1422 cm^-1 (Amide II)) in the whey-quercetin conjugates. Moreover, SDS-PAGE of conjugates produced by ultrasonication as well redox-pair method indicated shifting of protein bands slightly towards high molecular weight due to increase in the mass of proteins due

SUBMITTER: Baba WN 

PROVIDER: S-EPMC8515292 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature