Receptor-selective interleukin-4 mutein attenuates laser-induced choroidal neovascularization through the regulation of macrophage polarization in mice.

ABSTRACT: Macrophage polarization has been recognized as an important inflammatory regulator in choroidal neovascularization (CNV). However, the mechanisms regulating macrophage activation and polarization, as well as their effects on angiogenesis and CNV, have not yet been elucidated. IL-4 is implicated in macrophage activation and exerts different functions in various diseases through several receptors. In the current study, the effect of IL-4 muteins on CNV was investigated in vivo. CNV was induced by laser coagulation in wild type mice. IL-4 muteins were recombined into adenoviruses and injected into mice via the tail vein. To evaluate CNV, fluorescein fundus angiography and optical coherence tomography were performed on day 7 after coagulation. Quantitative PCR, western blotting and immu

SUBMITTER: Gao L 

PROVIDER: S-EPMC8515545 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature