AMPK promotes antitumor immunity by downregulating PD-1 in regulatory T cells via the HMGCR/p38 signaling pathway.

ABSTRACT:

Background

AMP-activated protein kinase (AMPK) is a metabolic sensor that maintains energy homeostasis. AMPK functions as a tumor suppressor in different cancers; however, its role in regulating antitumor immunity, particularly the function of regulatory T cells (Tregs), is poorly defined.

Methods

AMPKα1^fl/flFoxp3^YFP-Cre, Foxp3^YFP-Cre, Rag1^-/-, and C57BL/6 J mice were used for our research. Flow cytometry and cell sorting, western blotting, immuno-precipitation, immuno-fluorescence, glycolysis assay, and qRT-PCR were used to investigate the role of AMPK in suppressing programmed cell death 1 (PD-1) expression and for mechanistic investigation.

Results

The deletion of the AMPKα1 subunit in Tregs accelerates tumor growth by in

SUBMITTER: Pokhrel RH 

PROVIDER: S-EPMC8515644 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature