Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is one of the most intractable tumors in the world due to its high rate of recurrence and heterogeneity. Liver cancer initiating cells also called cancer stem cells (CSCs) play a critical role in resistance against typical therapy and high tumor-initiating potential. However, the role of the novel circular RNA (circRNA) circIPO11 in the maintenance of liver cancer initiating cells remains elusive.

Methods

CircRNAs highly conserved in humans and mice were identified from 3 primary HCC samples by circRNA array. The expression and function of circIPO11 were further evaluated by Northern blot, limiting dilution xenograft analysis, chromatin isolation by RNA purification-PCR assay (ChIRP) and HCC patient-derived tumor cells (PDC) models.

SUBMITTER: Gu Y 

PROVIDER: S-EPMC8515748 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature