Comparative study of immunohematological tests with canine blood samples submitted for a direct antiglobulin (Coombs') test.

ABSTRACT:

Background

A 2019 ACVIM consensus statement on diagnostics for immune-mediated hemolytic anemia (IMHA) in dogs made testing recommendations. As data on the performance of immunohematological tests was lacking, we undertook a comparative analysis.

Material and methods

Anticoagulated blood samples from 126 dogs suspected of having IMHA submitted to a diagnostic veterinary laboratory for a routine direct antiglobulin test (DAT) and from 28 healthy control dogs were evaluated for spherocytosis and autoagglutination before and after three saline washes. Samples were also subjected to different DATs: a gel minitube and an immunochromatographic strip kit used in clinics; neutral gel column cards, microtiter plates (at 4°, 22°, and 37°C), capillary tubes, and flow cytometry used in

SUBMITTER: Idalan N 

PROVIDER: S-EPMC8515749 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature