Reduction of autofluorescence in whole adult worms of Schistosoma japonicum for immunofluorescence assay.

ABSTRACT: Immunofluorescence assay is one of methods to understand the spatial biology by visualizing localization of biomolecules in cells and tissues. Autofluorescence, as a common phenomenon in organisms, is a background signal interfering the immunolocalization assay of schistosome biomolecules, and may lead to misinterpretation of the biomolecular function. However, applicable method for reducing the autofluorescence in Schistosoma remains unclear. In order to find a suitable method for reducing autofluorescence of schistosomes, different chemical reagents, such as Sudan black B (SBB), trypan blue (TB), copper sulfate (CuSO₄), Tris-glycine (Gly), and ammonia/ethanol (AE), at different concentrations and treatment time were tested, and SBB and CuSO₄ were verified for the ef

SUBMITTER: Wu Q 

PROVIDER: S-EPMC8515762 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature