Influence of the Hypercapnic Tumor Microenvironment on the Viability of Hela Cells Screened by a CO₂-Gradient-Generating Device.

ABSTRACT: Carbon dioxide (CO₂) levels outside of the physiological range are frequently encountered in the tumor microenvironment and laparoscopic pneumoperitoneum during clinical cancer therapy. Controversies exist regarding the biological effects of hypercapnia on tumor proliferation and metastasis concerning time frame, CO₂ concentration, and cell type. Traditional control of gaseous microenvironments for cell growth is conducted using culture chambers that allow for a single gas concentration at a time. In the present paper, Hela cells were studied for their response to varying levels of CO₂ in an aerogel-based gas gradient-generating apparatus capable of delivering a stable and quantitative linear CO₂ profile in spatial and temporal domains. Cells cul

SUBMITTER: Zhang S 

PROVIDER: S-EPMC8515822 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature