Examining Pathways of Iron and Sulfur Acquisition, Trafficking, Deployment, and Storage in Mineral-Grown Methanogen Cells.

ABSTRACT: Methanogens have a high demand for iron (Fe) and sulfur (S); however, little is known of how they acquire, deploy, and store these elements and how this, in turn, affects their physiology. Methanogens were recently shown to reduce pyrite (FeS₂), generating aqueous iron sulfide (FeS_aq) clusters that are likely assimilated as a source of Fe and S. Here, we compared the phenotypes of Methanococcus voltae grown with FeS₂ or ferrous iron [Fe(II)] and sulfide (HS^-). FeS₂-grown cells are 33% smaller yet have 193% more Fe than Fe(II)/HS^--grown cells. Whole-cell electron paramagnetic resonance revealed similar distributions of paramagnetic Fe, although FeS₂-grown cells showed a broad spectral feature attributed to intracel

SUBMITTER: Payne D 

PROVIDER: S-EPMC8516115 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature