Making a science out of preanalytics: An analytical method to determine optimal tissue fixation in real-time.

ABSTRACT: Modern histopathology is built on the cornerstone principle of tissue fixation, however there are currently no analytical methods of detecting fixation and as a result, in clinical practice fixation is highly variable and a persistent source of error. We have previously shown that immersion in cold formalin followed by heated formalin is beneficial for preservation of histomorphology and have combined two-temperature fixation with ultra-sensitive acoustic monitoring technology that can actively detect formalin diffusing into a tissue. Here we expand on our previous work by developing a predictive statistical model to determine when a tissue is properly diffused based on the real-time acoustic signal. We trained the model based on the morphology and characteristic diffusion curves of 30 ton

SUBMITTER: Bauer DR 

PROVIDER: S-EPMC8516200 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature