Interleukin enhancer-binding factor 2 promotes cell proliferation and DNA damage response in metastatic melanoma.

ABSTRACT:

Background

1q21.3 amplification, which is frequently observed in metastatic melanoma, is associated with cancer progression. Interleukin enhancer-binding factor 2 (ILF2) is located in the 1q21.3 amplified region, but its functional role or contribution to tumour aggressiveness in cutaneous melanoma is unknown.

Methods

In silico analyses were performed using the TCGA SKCM dataset with clinical annotations and three melanoma microarray cohorts from the GEO datasets. RNA in situ hybridisation and immunohistochemistry were utilised to validate the gene expression in melanoma tissues. Four stable melanoma cell lines were established for in vitro ILF2 functional characterisation.

Results

Our results showed that the ILF2 copy number variation (CNV) is positively correlated w

SUBMITTER: Zhang X 

PROVIDER: S-EPMC8516365 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature