Synthesis of Oligonucleotides Containing Trans Mitomycin C DNA Adducts at N⁶ of Adenine and N² of Guanine.

ABSTRACT: Mitomycin C, (MC), an antitumor drug, is a DNA alkylating agent currently used in the clinics. Inert in its native form, MC is reduced to reactive mitosenes, which undergo nucleophilic attack by guanine or adenine bases in DNA to form monoadducts as well as interstrand crosslinks (ICLs). Although ICLs are considered the most cytotoxic lesions, the role of each individual adduct in the drug's cytotoxicity is still not fully understood. Synthetic routes have been developed to access modified oligonucleotides containing dG MC-monoadducts and dG-MC-dG ICL at a single position of their base sequences to investigate the biological effects of these adducts. However, until now, oligonucleotides containing monoadducts formed by MC at the adenine base had not been available, thus preventing the exam

SUBMITTER: Zacarias O 

PROVIDER: S-EPMC8516704 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature