Single-cell transcriptome analysis identifies skin-specific T-cell responses in systemic sclerosis.

ABSTRACT:

Objectives

Although T cells have been implicated in the pathogenesis of systemic sclerosis (SSc), a comprehensive study of T-cell-mediated immune responses in the affected skin of patients with progressive SSc is lacking. Droplet-based single-cell transcriptome analysis of SSc skin biopsies opens avenues for dissecting patient-specific T-cell heterogeneity, providing a basis for identifying novel gene expression related to functional pathways associated with severity of SSc skin disease.

Methods

Single-cell RNA sequencing was performed by droplet-based sequencing (10x Genomics), focusing on 3729 CD3⁺ lymphocytes (867 cells from normal and 2862 cells from SSc skin samples) from skin biopsies of 27 patients with active SSc and 10 healthy donors. Confocal immunofluor

SUBMITTER: Gaydosik AM 

PROVIDER: S-EPMC8516708 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature