Culture-free biphasic approach for sensitive detection of Escherichia coli O157:H7 from beef samples.

ABSTRACT: Foodborne illnesses are a major threat to public health also leading to significant mortality and financial and reputational damage to industry. It is very important to detect pathogen presence in food products early, rapidly, and accurately to avoid potential outbreaks and economic loss. However, "gold standard" culture methods, including enrichment of pathogens, can take up to several days. Moreover, the food matrix often interferes with nucleic acid amplification methods of detection, requiring DNA extraction from the sample for successful molecular detection of pathogens. Here, we introduce a "biphasic" amplification method that can achieve high sensitivity detection with background noise from ground beef food samples without culture or other extraction methods in 2.5 h. Homogenized gr

SUBMITTER: Mostafa A 

PROVIDER: S-EPMC8516711 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature