Bile acids increase intestinal marker expression via the FXR/SNAI2/miR-1 axis in the stomach.

ABSTRACT:

Purpose

Intestinal metaplasia (IM) is a precancerous lesion that increases the risk of subsequent gastric cancer (GC) development. Previously, miR-1 has been shown to play an essential role in the initiation of bile acid (BA)-induced IM. The objective of the present study was to investigate the mechanism underlying miR-1 inhibition by BA in gastric cells.

Methods

Ingenuity pathway analysis (IPA) was used to identify molecules acting upstream of miR-1. The effects of deoxycholic acid (DCA), FXR and SNAI2 on the expression of intestinal markers were assessed using quantitative real-time PCR (qRT-PCR) and Western blotting. The expression level of major molecules was detected by immunohistochemistry (IHC) in tissue microarrays. The transcriptional regulation of miR-1 was verifie

SUBMITTER: Wang N 

PROVIDER: S-EPMC8516775 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature