Matrigel 3D bioprinting of contractile human skeletal muscle models recapitulating exercise and pharmacological responses.

ABSTRACT: A key to enhance the low translatability of preclinical drug discovery are in vitro human three-dimensional (3D) microphysiological systems (MPS). Here, we show a new method for automated engineering of 3D human skeletal muscle models in microplates and functional compound screening to address the lack of muscle wasting disease medication. To this end, we adapted our recently described 24-well plate 3D bioprinting platform with a printhead cooling system to allow microvalve-based drop-on-demand printing of cell-laden Matrigel containing primary human muscle precursor cells. Mini skeletal muscle models develop within a week exhibiting contractile, striated myofibers aligned between two attachment posts. As an in vitro exercise model, repeated high impact stimulation of contractions for 3 h

SUBMITTER: Alave Reyes-Furrer A 

PROVIDER: S-EPMC8516940 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature