Engineered mesenchymal stromal cell therapy during human lung ex vivo lung perfusion is compromised by acidic lung microenvironment.

ABSTRACT: Ex vivo lung perfusion (EVLP) is an excellent platform to apply novel therapeutics, such as gene and cell therapies, before lung transplantation. We investigated the concept of human donor lung engineering during EVLP by combining gene and cell therapies. Premodified cryopreserved mesenchymal stromal cells with augmented anti-inflammatory interleukin-10 production (MSC^IL-10) were administered during EVLP to human lungs that had various degrees of underlying lung injury. Cryopreserved MSC^IL-10 had excellent viability, and they immediately and efficiently elevated perfusate and lung tissue IL-10 levels during EVLP. However, MSC^IL-10 function was compromised by the poor metabolic conditions present in the most damaged lungs. Similarly, exposing cultured

SUBMITTER: Nykanen AI 

PROVIDER: S-EPMC8516994 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature