Snapshot multicolor fluorescence imaging using double multiplexing of excitation and emission on a single detector.

ABSTRACT: Fluorescence-based multispectral imaging of rapidly moving or dynamic samples requires both fast two-dimensional data acquisition as well as sufficient spectral sensitivity for species separation. As the number of fluorophores in the experiment increases, meeting both these requirements becomes technically challenging. Although several solutions for fast imaging of multiple fluorophores exist, they all have one main restriction; they rely solely on spectrally resolving either the excitation- or the emission characteristics of the fluorophores. This inability directly limits how many fluorophores existing methods can simultaneously distinguish. Here we present a snapshot multispectral imaging approach that not only senses the excitation and emission characteristics of the probed fluorophore

SUBMITTER: Dorozynska K 

PROVIDER: S-EPMC8517015 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature