Targeting interleukin-1 for reversing fat browning and muscle wasting in infantile nephropathic cystinosis.

ABSTRACT:

Background

Ctns^-/- mice, a mouse model of infantile nephropathic cystinosis, exhibit hypermetabolism with adipose tissue browning and profound muscle wasting. Inflammatory cytokines such as interleukin (IL)-1 trigger inflammatory cascades and may be an important cause for cachexia. We employed genetic and pharmacological approaches to investigate the effects of IL-1 blockade in Ctns^-/- mice.

Methods

We generated Ctns^-/- Il1β^-/- mice, and we treated Ctns^-/- and wild-type control mice with IL-1 receptor antagonist, anakinra (2.5 mg/kg/day, IP) or saline as vehicle for 6 weeks. In each of these mouse lines, we characterized the cachexia phenotype consisting of anorexia, loss of weight, fat mass and lean mass, elevation of m

SUBMITTER: Cheung WW 

PROVIDER: S-EPMC8517356 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature