Nuclear localization dictates hepatocarcinogenesis suppression by glycine N-methyltransferase.

ABSTRACT:

Background

GNMT (glycine N-methyltransferase) is a tumor suppressor gene, but the mechanisms mediating its suppressive activity are not entirely known.

Methods

We investigated the oncosuppressive mechanisms of GNMT in human hepatocellular carcinoma (HCC). GNMT mRNA and protein levels were evaluated by quantitative RT-PCR and immunoblotting. GNMT effect in HCC cell lines was modulated through GNMT cDNA induced overexpression or anti-GNMT siRNA transfection.

Results

GNMT was expressed at low level in human HCCs with a better prognosis (HCCB) while it was almost absent in fast-growing tumors (HCCP). In HCCB, the nuclear localization of the GNMT protein was much more pronounced than in HCCP. In Huh7 and HepG2 cell lines, GNMT forced expression inhibited the proliferation

SUBMITTER: Simile MM 

PROVIDER: S-EPMC8517931 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature