Accelerating Reaction Rates of Biomolecules by Using Shear Stress in Artificial Capillary Systems.

ABSTRACT: Biomimetics is a design principle within chemistry, biology, and engineering, but chemistry biomimetic approaches have been generally limited to emulating nature's chemical toolkit while emulation of nature's physical toolkit has remained largely unexplored. To begin to explore this, we designed biophysically mimetic microfluidic reactors with characteristic length scales and shear stresses observed within capillaries. We modeled the effect of shear with molecular dynamics studies and showed that this induces specific normally buried residues to become solvent accessible. We then showed using kinetics experiments that rates of reaction of these specific residues in fact increase in a shear-dependent fashion. We applied our results in the creation of a new microfluidic approach for the mult

SUBMITTER: Hakala TA 

PROVIDER: S-EPMC8517977 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature