Charting the complexity of the activated sludge microbiome through a hybrid sequencing strategy.

ABSTRACT:

Background

Long-read sequencing has shown its tremendous potential to address genome assembly challenges, e.g., achieving the first telomere-to-telomere assembly of a gapless human chromosome. However, many issues remain unresolved when leveraging error-prone long reads to characterize high-complexity metagenomes, for instance, complete/high-quality genome reconstruction from highly complex systems.

Results

Here, we developed an iterative haplotype-resolved hierarchical clustering-based hybrid assembly (HCBHA) approach that capitalizes on a hybrid (error-prone long reads and high-accuracy short reads) sequencing strategy to reconstruct (near-) complete genomes from highly complex metagenomes. Using the HCBHA approach, we first phase short and long reads from the highly compl

SUBMITTER: Liu L 

PROVIDER: S-EPMC8518188 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature