Genetic and process engineering strategies for enhanced recombinant N-glycoprotein production in bacteria.

ABSTRACT:

Background

The production of N-linked glycoproteins in genetically amenable bacterial hosts offers great potential for reduced cost, faster/simpler bioprocesses, greater customisation, and utility for distributed manufacturing of glycoconjugate vaccines and glycoprotein therapeutics. Efforts to optimize production hosts have included heterologous expression of glycosylation enzymes, metabolic engineering, use of alternative secretion pathways, and attenuation of gene expression. However, a major bottleneck to enhance glycosylation efficiency, which limits the utility of the other improvements, is the impact of target protein sequon accessibility during glycosylation.

Results

Here, we explore a series of genetic and process engineering strategies to increase recombinant N-lin

SUBMITTER: Pratama F 

PROVIDER: S-EPMC8518210 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature