HBeAg mediates inflammatory functions of macrophages by TLR2 contributing to hepatic fibrosis.

ABSTRACT:

Background

We and others have confirmed activation of macrophages plays a critical role in liver injury and fibrogenesis during HBV infection. And we have also proved HBeAg can obviously induce the production of macrophage inflammatory cytokines compared with HBsAg and HBcAg. However, the receptor and functional domain of HBeAg in macrophage activation and its effects and mechanisms on hepatic fibrosis remain elusive.

Methods

The potentially direct binding receptors of HBeAg were screened and verified by Co-IP assay. Meanwhile, the function domain and accessible peptides of HBeAg for macrophage activation were analyzed by prediction of surface accessible peptide, construction, and synthesis of truncated fragments. Furthermore, effects and mechanisms of the activation of hepa

SUBMITTER: Xie X 

PROVIDER: S-EPMC8518250 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature