Identification of genetic determinants of tomato brown rugose fruit virus that enable infection of plants harbouring the Tm-2² resistance gene.

ABSTRACT: Tomato cultivars containing the Tm-2² resistance gene have been widely known to resist tobacco mosaic virus (TMV) and tomato mosaic virus. Tomato brown rugose fruit virus (ToBRFV), a new emerging tobamovirus, can infect tomato plants carrying the Tm-2² gene. However, the virulence determinant of ToBRFV that overcomes the resistance conferred by the Tm-2² gene remains unclear. In this study, we substituted the movement protein (MP) encoding sequences between ToBRFV and TMV infectious clones and conducted infectivity assays. The results showed that MP was the virulence determinant for ToBRFV to infect Tm-2² transgenic Nicotiana benthamiana plants and Tm-2² -carrying tomato plants. A TMV MP chimera with amino acid residues 60-186 of ToBRF

SUBMITTER: Yan ZY 

PROVIDER: S-EPMC8518564 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature