Rapid genotyping of tilapia lake virus (TiLV) using Nanopore sequencing.

ABSTRACT: Infectious diseases represent one of the major challenges to sustainable aquaculture production. Rapid, accurate diagnosis and genotyping of emerging pathogens during early-suspected disease cases is critical to facilitate timely response to deploy adequate control measures and prevent or reduce spread. Currently, most laboratories use PCR to amplify partial pathogen genomic regions, occasionally combined with sequencing of PCR amplicon(s) using conventional Sanger sequencing services for confirmatory diagnosis. The main limitation of this approach is the lengthy turnaround time. Here, we report an innovative approach using a previously developed specific PCR assay for pathogen diagnosis combined with a new Oxford Nanopore Technologies (ONT)-based amplicon sequencing method for pathogen ge

SUBMITTER: Delamare-Deboutteville J 

PROVIDER: S-EPMC8518713 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature