The human WNT5A isoforms display similar patterns of expression but distinct and overlapping activities in normal human osteoblasts.

ABSTRACT: WNT5A activates noncanonical Wnt signaling pathways and has critical functions in early development, differentiation, and tissue homeostasis. Two major WNT5A protein isoforms, which in this study we term WNT5A-L(A) and WNT5A-S(B), have been identified that differ by 18 AA at their amino terminus. Functional differences between the isoforms have been indicated in studies utilizing cancer cell lines but the activities of the isoforms in normal cells and during differentiation have not been explored. We examined the WNT5A isoforms in the normal osteoblast cell line hFOB1.19. WNT5A-L(A) and WNT5A-S(B) transcripts increased from Days 3 to 21 of differentiation but WNT5A-S(B) showed a greater fold-change. In undifferentiated cells, there are 2-fold more WNT5A-L(A) than WNT5A-S(B) transcripts. To

SUBMITTER: Bhandari D 

PROVIDER: S-EPMC8518764 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature