Functional role of brain-engrafted macrophages against brain injuries.

ABSTRACT:

Background

Brain-resident microglia have a distinct origin compared to macrophages in other organs. Under physiological conditions, microglia are maintained by self-renewal from the local pool, independent of hematopoietic progenitors. Pharmacological depletion of microglia during whole-brain radiotherapy prevents synaptic loss and long-term recognition memory deficits. However, the origin or repopulated cells and the mechanisms behind these protective effects are unknown.

Methods

CD45^low/int/CD11b⁺ cells from naïve brains, irradiated brains, PLX5622-treated brains and PLX5622 + whole-brain radiotherapy-treated brains were FACS sorted and sequenced for transcriptomic comparisons. Bone marrow chimeras were used to trace the origin and long-term morpholog

SUBMITTER: Feng X 

PROVIDER: S-EPMC8520231 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature