Citrulline deiminase pathway provides ATP and boosts growth of Clostridium carboxidivorans P7.

ABSTRACT:

Background

Clostridium carboxidivorans P7 is capable of producing ethanol and butanol from inexpensive and non-food feedstock, such as syngas. Achieving improved ethanol and butanol production in the strain for industrial application depends on the energetics and biomass, especially ATP availability.

Results

This study found that exogenous addition of citrulline promoted accumulation of ATP, increased specific growth rate, and reduced the doubling time of C. carboxidivorans P7. In heterotrophic fermentation experiments, the addition of citrulline increased intracellular ATP by 3.39-fold, significantly enhancing the production of total alcohol (ethanol + butanol) by 20%. Moreover, in the syngas fermentation experiments, the addition of citrulline improved the level of intrace

SUBMITTER: Li X 

PROVIDER: S-EPMC8520249 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature